Y514-A self-cleaning chlorophyll sensor calibration

Writer： admin Time：2023-12-06 14:46:24 Browse：215℃

Y514-A self-cleaning chlorophyll sensor calibration

Required equipments and raw materials
Required equipments: two 1L brown bottles, one iron stand, one set of gloves
Raw materials: 2mg/L chlorophyll standard solution and deionized water

Calibration steps
There are three methods of user calibration for the chlorophyll sensor: 0-point calibration, 1-point calibration and 2-points calibration.
0-point calibration was done in deionized water, 1-point calibration was done in 2mg/L chlorophyll standard solution, 2-points calibration was done in deionized water and 2mg/L chlorophyll standard solution.

0-point calibration step (change the B value)

Step 1: put an appropriate amount of distilled water or deionized water into a 1L brown bottle, remove the rubber protective cap on the front of the sensor and put the sensor in the center of the brown bottle, which not close to the wall around the brown bottle, and then fix the sensor with an iron stand, adjust the high and low position of the sensor. Pay attention to maintain a distance greater than 10cm between the front end of the sensor and the bottom of the brown bottle.
Step 2: Under the “Automatic Calibration” interface, click “0 point” to do zero-point calibration, wait for the values in the pop-up window to stabilize, and click “OK” to complete the calibration. Click “Get” to check whether the B value is written correctly.
Step 3: click “Start” under the “CHL” interface to get the real-time measurement of the CHL and temperature, check that the CHL is close to 0 to verify that the zero-point calibration is successful.

1-point calibration step (change the K value)

Step 1: put an appropriate amount of 2mg/L chlorophyll standard solution into a 1L brown bottle, remove the rubber protective cap on the front of the sensor and put the sensor in the center of the brown bottle, which not close to the wall around the brown bottle, and then fix the sensor with an iron stand, adjust the high and low position of the sensor. Pay attention to maintain a distance greater than 10cm between the front end of the sensor and the bottom of the brown bottle.
Step 2: Under the “Automatic Calibration” interface, change the “Single/First Point Calibration Value” to 227.1, click “1 point” to do one-point calibration, wait for the values in the pop-up window to stabilize, and click “OK” to complete the calibration. Click “Get” to check whether the K value is written correctly.
Step 3: click “Start” under the “CHL” interface to get the real-time measurement of the CHL and temperature, check that the CHL is close to 227.1 to verify that the one-point calibration is successful.

2-points calibration step (change the K and B values)

Step 1: put an appropriate amount of distilled water or deionized water into a 1L brown bottle, and put an appropriate amount of 2mg/L chlorophyll standard solution into another 1L brown bottle.
Step 2: remove the rubber protective cap on the front of the sensor, put the sensor in the center of the brown bottle containing distilled water or deionized water, which not close to the wall around the brown bottle, and then fix the sensor with an iron stand, adjust the high and low position of the sensor. Pay attention to maintain a distance greater than 10cm between the front end of the sensor and the bottom of the brown bottle.
Step 3: Under the “Automatic Calibration” interface, change the “Single/First Point Calibration Value” to 0, change the “Second Point Calibration Value” to 227.1, click “2 points” to do two-points calibration, wait for the values in the pop-up window to stabilize, and click “OK”. Then wipe the sensor dry and put it into 2mg/L chlorophyll standard solution according to the method of step 2, wait for the values in the pop-up window to stabilize, and click “OK” to complete the calibration. Click “Get” to check whether the K and B values are written correctly;
Step 4: click “Start” under the “CHL” interface to get the real-time measurement of the CHL and temperature, check that the CHL is close to 227.1, then clean the sensor with distilled water or deionized water, wipe it dry and put it into the distilled water or deionized water according to the method of step 2 to get the real-time measurement of the CHL and temperature, check that the CHL is close to 0 to verify that the two-points calibration is successful.